Histone lysine demethylase KDM5B facilitates proliferation and suppresses apoptosis in human acute myeloid leukemia cells through the miR-140-3p/BCL2 axis

Identification of miR-140-3p as a downstream target of KDM5B in human AML cells. (A) Heatmap of the differential expression of miRNAs (P < 0.05) in control and KDM5B KD MOLM-13 cells. (B–D) The expression level of miR-140-3p was determined in KDM5B KD MOLM-13 (B), MOLM-14 (C), and MV-4-11 cells (D). (E) The expression level of miR-140-3p in KDM5B overexpressed MOLM-13 cells. (F) The expression levels of indicated genes in control and KDM5B KD MOLM-13 cells. (G) Evaluation of H3K4me3 enrichment on target genes by ChIP-qPCR in control and KDM5B KD MOLM-13 cells. (H,I) Evaluation of KDM5B binding on target genes by CUT&Tag-qPCR in control and KDM5B KD MOLM-13 (H) and MOLM-14 (I) cells. Quantification from three biological repeats. Data represent the mean + SD. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001, by Student's t-test.