Silencing KDM5B activates the interferon response genes in MOLM-13 cells. (A,B) mRNA-sequencing analysis of MOLM-13 cells upon KDM5B KD. (A) Heatmap of differential expression (fold change >2) genes. (B) KEGG pathway analysis of the 131 up-regulated genes upon KDM5B KD. (C) Relative mRNA levels of indicated genes were checked by RT-qPCR and normalized to Actb. Data were generated from three biological repeats. Primers are provided in Supplemental Table S2. The P-value of significantly affected genes is marked. (D) Immunoblotting of indicated proteins in control and KDM5B KD MOLM-13 cells. (Right) Statistical analysis from three biological repeats. Data represent the mean + SD (C,D). (*) P < 0.05 and (**) P < 0.01, by Student's t-test.
