Dynamic of alternative poly(A) site usage. (A) PCA resulting from A-seq2 read coverage over the final peak set using naive, all 48 h activated, memory, and regulatory T cells. (B) Number of shortening and lengthening events identified in each comparison. Dark color indicates higher numbers, light color indicates lower numbers. (C) Functional annotations of genes associated to an APA event between activated two divisions and either memory or regulatory states. (D) Example of 3′-UTR Arih2 gene (chr9:108,602,316–108,606,119, “−”) showing reverted length during activation and effector stages. (E) Concordance of APA sites’ usage in genes associated with a reverting event. (F) Venn diagram showing overlap of genes presenting a reverting pattern. (G) Functional annotations of genes associated with a reverted APA event.
