Spliceosomal helicases DDX41/SACY-1 and PRP22/MOG-5 both contribute to proofreading against proximal 3′ splice site usage

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FIGURE 3.
FIGURE 3.

Overview of WT versus sacy-1(G533R) RNA-seq comparative splicing analysis. (A) Categories of alt. splicing events. Two hundred and eleven events are divided and subdivided into specific categories by the features described in the box (see Materials and Methods). (B) Sequence logo. The height of each base represents significant enrichment of nucleotide identity at that position over random chance, created on WebLogo (https://weblogo.berkeley.edu/) (Crooks et al. 2004). Note that for the sacy-1(G533R) proximal and distal sites, only the 206 events for which two adjacent sites were used could have their splice sites categorized as proximal or distal by definition.

This Article

  1. RNA 30: 404-417