SACY-1 depletion increases the usage of proximal splice sites that poorly match the C. elegans consensus sequence. (A) Histogram. X-axis: relative position of the splice site that increases usage upon SACY-1 depletion compared to the other site of the pair; a negative number indicates shift in the 5′ (upstream or proximal) direction. (B) Sequence logo. The height of each base represents significant enrichment of nucleotide identity at that position over random chance, created on WebLogo (https://weblogo.berkeley.edu/) (Crooks et al. 2004). T is used rather than U because these splice site sequences are inferred from alignments with the genome rather than directly sequenced.
