TABLE 5.
Trigger sequences for Figure 6 with contrast and relative Fmax
| Trigger identity | Sequence | Contrasta | Fmaxb |
|---|---|---|---|
| RNA (WT) | GGG UUA GCC AGG CUA CGU GCU AUU GUC C | 96 ± 42 | 219 ± 3 |
| DNA | GGG TTA GCC AGG CTA CGT GCT ATT GTC C | 92 ± 8 | 126 ± 8 |
| Reverse RNA (+GGG) | GGG CCU GUU AUC GUG CAU CGG ACC GAU UGG G | 1.6 ± 0.2 | 2.3 ± 0.3 |
| dsRNA | WT + reverse complement | 2.4 ± 1.3 | 5.5 ± 2.4 |
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aObtained by taking the average of the fluorescence values at t = 243–247 min and dividing this by the average of t = 65–69 min, which are the fluorescent values immediately before the trigger was added at 70 min.
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bObtained by taking the average of the fluorescence values at t = 243–247 min. The errors are from the standard deviation of the triplicate data.
