Turn-on RNA Mango Beacons for trans-acting fluorogenic nucleic acid detection

TABLE 4.

Mango Beacon on-rates

Identity # Nucleotides of hybridizationa On-rate Kon (M−1 sec−1)
MIIB + 4i 25 1.2 × 104 ± 1.0 × 103
MIIB + DNA inhibition (5D) 20 1.9 × 104 ± 1.2 × 103
Molecular beacon (Tsourkas et al. 2003)b 17–19 4.10 × 103 to 7.4 × 103
Linear dual labeled probe (Tsourkas et al. 2003)c 17–19 1.0 × 104 to 1.4 × 104
  • aThe number of nucleotides of hybridization to target.

  • bRange of six reported rates, no error reported.

  • cRange of three reported rates, no error reported.

This Article

  1. RNA 30: 392-403