TABLE 3.

Trigger sequences for Supplemental Figures S3 and S5 with contrast and relative F_max

Identity	Sequence	Contrast	F_max
MIIB + 4i Construct 2	GGA CAA TAG CAG GAG AGG AGA GGA AGA GGA GAA CGT AGC CTG CTC CGC TCC	n/a	n/a
Trigger A (WT)	GGG UUA GCC AGG CUA CGU GCU AUU GUC C	n/a	n/a
Trigger B	GGG UUA GCC CCA GGC UAC GUG CUA UUG UCC	n/a	n/a
Trigger C	GGG UUA GCC CAG GCU ACG UGC UAU UGU CC	n/a	n/a
MIIB + 4i	GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CGC UAA	85 ± 23	33 ± 3
C20A	GGG UUA GCC AGG CUA CGU GAU AUU GUC C	113 ± 22	191 ± 11
C20U	GGG UUA GCC AGG CUA CGU GUU AUU GUC C	72 ± 22	130 ± 5
U21G	GGG UUA GCC AGG CUA CGU GCG AUU GUC C	79 ± 8	96 ± 7
U23G	GGG UUA GCC AGG CUA CGU GCU AGU GUC C	117 ± 7	130 ± 2

Residues in bold indicate mutations. Trigger sequences are shown starting with 5′ end.

Turn-on RNA Mango Beacons for trans-acting fluorogenic nucleic acid detection