TABLE 2.
Mango Beacon and trigger sequences with contrast and Fmax
| Identity | Type | Sequence | Contrast | Fmax |
|---|---|---|---|---|
| Beacon MI.B2 Trigger MI.B2 |
MIB + 4i | UUG CAC AUG CCG AGG GAC GG UGC GG AGA GG AGA GAG CCG UUG U CUCC ACG ACC AGC GC AAC GUG UAC GGC CUC GGC AAC AGC UGC UGG UCG CGG G |
107 | 321a |
| Beacon MI.B3 Trigger MI.B3 |
MIB + 4i | CGA CCA GCG CAG AGG GAC GG UGC GG AGA GG AGA CGA UAU CGU CAU CUCC A UGG C GCU GGU CGC GUC GCU AUA GCA GUA GGU ACC GGG |
91 | 261a |
| Beacon MI.B4 Trigger MI.B4 |
MIB + 4i | ACU UCA GGG UCA AGG GAC GG UGC GG AGA GG AGA GGA UAC CUC UCU CUCC CUC UGG GCC UCG UGA AGU CCC AGU CCU AUG GAG AGA ACG AGA CCC GGA GCG G |
60 | 169a |
| Beacon MI.B5 Trigger MI.B5 |
MIB + 4i | AAG GGU GUA AAA AGG GAC GG UGC GG AGA GG AGA CGC AGC UCA GUA CUCC A GUC CGC CUA UUC CCA CAU UUU GCG UCG AGU CAU UGU CAG GCG GAUGG |
84 | 210a |
| Beacon MII.CBA1 Trigger MII.CBA1 |
MIIB + 4i | GGA UCC UGA GUC AGG AGA GGA GAG GAA GAG GAG AAA GCG CCA AA CUCC UAG AAA AAA CA UAG GAC UCA GUU CGC GGU UUU CUU UUU UGU GG |
115 | 149 |
| Beacon MII.CBA2 Trigger MII.CBA2 |
MIIB + 4i | GGC AUA CCG GAG AGG AGA GGA GAG GAA GAG GAG ACC AUU GUC AA CUCC UCA ACG AGC GC GUA UGG CCU CGG UAA CAG UUG UUG CUC GCG GG |
45 | 142 |
| Beacon MII.O1 Trigger MII.O1 |
MIIB + 4i | GGA AAA GCG GAA GGA GAG GAG AGG AAG AGG AGA AAA GUU UGA CUCC UA GAG AA UUU UCG CCU UUU CAA ACU UCU CUU CAA AAG UCG ACG AAC GCG AAU AGG |
0.24 | 19 |
| Beacon MII.O2 Trigger MII.O2 |
MIIB + 4i | GGA AAA GCG GAA AAA GGA GAG GAG AGG AAG AGG AGA GUU UGA AGA GAA GUU CUCCUU UCA GCU GCU UGC GCU U UUU UCG CCU UUU CAA ACU UCU CUU CAA AAG UCG ACG AAC GCG AAU AGG |
53 | 211a |
| Beacon MII.O4 Trigger MII.O4 |
MIIB + 4i | GGC GCA UUU ACG CUG AGG AGA GGA GAG GAA GAG GAG AGC UUG CUG GUA GAA ACU CCU UUG UUG AGA U CCG CGU AAA UGC GAC CGA ACG ACC AUC UUU AAC AAC UCU AGG |
37 | 787a |
| Beacon MII.HBA1 Trigger MII.HBA1 |
MIIB + 4i | GGA CUU CAG GGU GAA GGA GAG GAG AGG AAG AGG AGA GGA UGC CUC UCU CUCC UC UCU GGG CCU CG UGA AGU CCC ACU CCU ACG GAG AGA ACG AGA CCC GGA GCG G |
275 | 217 |
| Beacon MII.HBA2 Trigger MII.HBA2 |
MIIB + 4i | GGC AAU GAU CUU AGG AGA GGA GAG GAA GAG GAG AGA UCU UCA UU CUCC UGU GCU GGG UG GUU ACU AGA ACU AGA AGU AAC ACG ACC CAC GG |
3 | 4 |
| Beacon MII.HBA3 Trigger MII.HBA3 |
MIIB + 4i | GGC CGA CUG CUG AGG AGA GGA GAG GAA GAG GAG AUC ACC UUC AC CUCC UCG UUC CAG UU GGC UGA CGA CAG UGG AAG UGG CAA GGU CAA GG |
195 | 214 |
| Beacon MII.HBAZ1 Trigger MII.HBAZ1 |
MIIB + 4i | GGG AAA GGG UGU AAA GGA GAG GAG AGG AAG AGG AGA CGC AAC UAA GUC CUCC UU CCG CCU A CUU UCC CAC AUU GCG UUG AUU CAG AGG CGG AUG G |
40 | 205 |
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MI and MII denote either a Mango I- or Mango II-based Beacon reporter. Beacon sequences are shown starting with 5′ end, trigger sequences are shown starting with 3′ end.
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aThe cuvette used had a 5× larger path length. Reactions were performed in singlet—no errors are presented. Trigger sequences are bolded. Fmax here represents the final fluorescence following between 2 and 4 h of incubation with the trigger. These Beacons were designed to target various RNA from Mouse, Drosophila, Chicken, and Human by varying sequences of the hybridization arms.
