Self-inhibiting, unimolecular Mango Beacon and trigger sequences for Figure 5 with contrast and relative Fmax
| Identity | Sequence | Contrasta | Fmaxb |
|---|---|---|---|
| MIB | GGA CAA UAG CAG GGA CGG UGC GGA GAG GAG AAC GUA GCC U | 23 ± 6 | 38 ± 3 |
| MIB + 4i | GGA CAA UAG CAG GGA CGG UGC GGA GAG GAG AAC GUA GCC UGC UCC GCU AA | 85 ± 23 | 33 ± 3 |
| MIIB | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CU | 2.2 ± 0.2 | 159 ± 6 |
| MIIB + 4i | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CGC UAA | 78 ± 10 | 252 ± 3 |
| MIIB + 5i | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CUG CUA A | 234 ± 24 | 181 ± 5 |
| MIIB + 6i | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CUC GCU AA | 138 ± 51 | 183 ± 8 |
| MIIB + 7i | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CUC UGC UAA | 163 ± 15 | 168 ± 10 |
| MIIB + 8i | GGA CAA UAG CAG GAG AGG AGA GGA AGA GGA GAA CGU AGC CUG CUC CUC UUG CUA A | 57 ± 13 | 76 ± 2 |
| Trigger | GGG UUA GCC AGG CUA CGU GCU AUU GUC C | n/a | n/a |
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Adding the inhibiting domain increases the contrast ratio by decreasing the “OFF” state fluorescence values. Nucleotides in bold indicate Mango core sequences with the additional flanking As; nucleotides that are in bold and italicized indicate inhibitory nucleotides (+ ni); underlined regions indicate potential core nucleotides to which a region in one of the flanking arms hybridizes. Trigger sequences are shown starting with 5′ end.
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aObtained by taking the average of the fluorescence values at t = 238–240 min and dividing it by the average of t = 67–69 min, which are the fluorescent values immediately before the trigger was added at 70 min.
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bObtained by taking the average of the fluorescence values at t = 238–240 min. The errors are from the standard deviation of the triplicate data.
