FIGURE 5.
Contrast and fluorescence optimization of self-inhibiting domains. (A) Fluorescence emergence (measured as described previously) of self-inhibiting unimolecular Mango Beacons with increasing base pairs of self-inhibition (n = 4–8). Note, MIIB + 4i is a replicate of the data presented in Figure 4B. (B) Prefolding region of plot A is highlighted for clarity. (C) Contrast observed for self-inhibiting Mango Beacons, where n = 0 is equivalent to the MIIB construct. (D) Maximum fluorescence intensity observed for self-inhibiting Mango Beacons, where n = 0 is equivalent to the MIIB construct.
