Turn-on RNA Mango Beacons for trans-acting fluorogenic nucleic acid detection

Schematic for the development of Mango Beacon structures (MIB/MIIB). Starting with the wild-type Mango RNA aptamer, removing the stem–loop results in a minimal Mango core with marginally reduced fluorescence. The addition of hybridization arms results in sequence-targeting Mango Beacon constructs, but with variable inhibition of G-quadruplex core folding efficiency. The GAA^A loop adapter found in Mango I and II is indicated in purple.