Fluorescence emergence strategies for MBs and novel Mango Beacons. (A) Fluorescence emergence in a conventional MB, triggered by binding to a complementary target sequence, which separates the chemically conjugated fluorophore (green circle) and quencher (gray square). (B) Bimolecular Mango Beacon formed by appending target binding hybridization arms (blue) onto a minimal Mango I or II core (black). Folding of the beacon is inhibited by a DNA suppressor (red), which is released upon binding to a trigger sequence (orange), allowing for the Mango core to fold and bind to its ligand TO1-Biotin (green). (C) Simple unimolecular Mango Beacon, which shows minimal folding until the addition of a trigger. (D) Self-inhibited unimolecular Mango Beacon, which has an inhibition domain (red) that deliberately hybridizes to the Mango core, reducing folding in the absence of a trigger.
