Characterization of tRNA splicing enzymes RNA ligase and tRNA 2′-phosphotransferase from the pathogenic fungi Mucorales

Mucor RNL is inactive in vivo when paired with the T4 end-healing enzyme Pnkp. The S. cerevisiae trl1Δ p(CEN URA3 TRL1) “shuffle” strain was transformed with marked plasmids expressing the indicated genes and with empty vector controls. Individual transformants were streaked on agar medium containing FOA, which was photographed after incubation for 10 d at 30°C. Two independent trl1Δ p(CEN URA3 TRL1) MciRNL + T4PNKP isolates were tested for growth on FOA.