Beta distributions model inclusion level differences across multiple groups. (A) Scatterplot comparing betAS’ difference of the median of absolute differences between and within groups and the estimated probability that absolute differences between groups are greater than those within groups. Points are colored based on the estimated F-statistic as the median of absolute differences between groups divided by the median of absolute differences within groups. Highlighted points are associated with the selected example AS events in panels C–F. (B) Scatterplot comparing betAS’ difference of the median of absolute differences between and within groups and the estimated F-statistic as the median of absolute differences between groups divided by the median of absolute differences within groups. Points are colored based on the estimated probability that absolute differences between groups are greater than those within groups. Highlighted points are associated with the selected examples in panels C–F. (C–F, left) density plots of the absolute differences between (red) and within (gray), with dashed vertical lines indicative of the median values of each distribution. (Right) Beta distributions (violin plots of the emitted values) for selected events, identified by vast-tools’ IDs (VAST-DB annotation for the human hg19 genome assembly): (C) HsaEX0021044 (gene DST, chr6:56329483–56329554), (D) HsaEX0015811 (gene CLTB, chr5:175823480–175823533), (E) HsaEX0051256 (gene PTS, chr11:112100931–112100953), (F) HsaEX0055568 (gene RPS24, chr10:79799962–79799983).
