JTE-607 impairs cell cycle progression by inducing S-phase arrest. (A, B) Cell cycle distribution and quantification of HPNE, MiaPaCa2, and Panc1 cell lines treated with 1–10 µM JTE-607. (*) P < 0.05, (**) P < 0.001, (***) P < 0.0001, two-way ANOVA with Dunnett's multiple comparisons test. (C, D) Cell cycle distribution and quantification of HPNE and Panc1 cell lines upon transient CPSF3 knockdown by siRNA after 24 h of transfection. (siCTL) Nontargeting control siRNA. (*) P < 0.01, (**) P < 0.001, two-way ANOVA with Dunnett's multiple comparisons test. Quantification in B and D is the number of cells in the S-phase. (E) BrdU incorporation assay showing cell cycle population upon JTE-607 treatment. The lower left quadrant represents the G1 population. The lower right quadrant represents the G2 population. The top two quadrants represent S-phase populations; early S-phase (left) and late S-phase (right).
