JTE-607 induces chromatin instability selectively in PDAC cells. (A) MNase assay of Panc1 cells treated with 10 µM JTE-607 or 1 µM CBL0137. (B) MNase assay of immortalized HPNE control cells treated with the CPSF3 inhibitor JTE-607 (10 µM) or CBL0137 (1 µM). (C) GFP + HeLa-TI cells following 10 µM JTE-607 or 1 µM CBL0137 treatment. (D) Fold change of GFP + HeLa-TI from C. (***) P < 0.0001; two-way ANOVA with Tukey's multiple comparisons test. (E) Flow cytometry analysis of GFP + HeLa-TI cells following 10 µM JTE-607 or 1 µM CBL0137 treatment. Fold change is shown as mean ± SEM of two independent experiments. (**) P < 0.01, (***) P < 0.0001, ordinary one-way ANOVA with Tukey's multiple comparisons test.
