CPSF3 inhibition blocks pancreatic cancer cell proliferation through disruption of core histone mRNA processing

JTE-607 induces RD histone transcriptional readthrough. (A, B) Quantification of RD histone readthrough in Panc1 and HPNE cells after 24 h (A) and 2 h (B) of 10 µM JTE-607 treatment by RT-qPCR. Data were normalized to DMSO controls (dashed horizontal line). (*) P < 0.05, (**) P < 0.01, (***) P < 0.001; two-way ANOVA with Sidak's multiple comparisons test. (C, D) Quantification of RI histone readthrough in Panc1 and HPNE cells after 24 h (C) and 2 h (D) of 10 µM JTE-607 treatment by RT-qPCR. Data were normalized to DMSO controls (dashed horizontal line). (*) P < 0.05, (**) P < 0.01, (***) P < 0.001; two-way ANOVA with Sidak's multiple comparisons test.