JTE-607 decreases gene expression of RD histones. (A) Heatmap of top differentially expressed genes after 24 h of 10 µM JTE-607 treatment. RD histones are colored in blue. Expression is plotted as transformed expression value. (B) DSeq2 normalized counts of H3F3A and H2AZ1 histone variants (RI) in Panc1 cells treated with 10 µM JTE-607 for 24 h. (**) P < 0.001. (C) mRNA expression of H2B (HIST1H2BC) and H3 (HIST1H3B) in MiaPaCa2 cells treated with JTE-607. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, ordinary one-way ANOVA with Dunnett's multiple comparisons test. (D, E) Survival analyses of low (blue) and high (red) expression of the RD histone signature (50 genes) in the TCGA-PAAD data set. Signature genes were uploaded to GEPIA2 to assess disease-free (D) and overall survival (E) based on median.
