PDAC cell lines are sensitive to CPSF3 inhibition by JTE-607. (A) IC50 of JTE-607 on immortalized control (HPNE and HPDE) and PDAC (MiaPaCa2, Panc1, Suit2, BxPC3) cell lines after 72 h of treatment. (B) IC50 of JTE-607 on human fibroblast C7 and PancPat CAFs after 72 h of treatment. (C) Association between doubling time and IC50 of JTE-607 in pancreatic cell lines. Red denotes PDAC cells while black denotes immortalized control cell lines. R2 = 0.4995. (D, E) Proliferation rates at days 0, 2, 4, and 6 of immortalized control and PDAC cell lines after treatment with escalating concentrations of JTE-607. (*) P < 0.05; two-way ANOVA with Dunnett's multiple comparisons test. Data are shown as mean ± SEM. (F) Clonogenic growth assay of PDAC cell lines after treatment with increasing concentration of JTE-607.
