CPSF3 is highly expressed in PDAC and is required for PDAC cell proliferation. (A) CPSF3 mRNA expression from CPTAC PDAC patient data. Whiskers indicate minimum and maximum data points. (***) P < 0.0001, ordinary one-way ANOVA with Tukey multiple comparisons test. (B) CPSF3 mRNA expression from PDAC patient data (TCGA) as compared to normal pancreas (GTEx). Whiskers indicate minimum and maximum data points. (***) P < 0.0001, unpaired t-test with Welch's correction. (C) Immunoblot of CPSF3 in immortalized control pancreatic epithelial cells (black) and PDAC cells (red). (D) Kaplan–Meier survival curves of PDAC patients with high (red) and low (blue) CPSF3 mRNA levels. Data were obtained from the CPTAC database. (E) Immunoblot of CPSF3 in shNTC, sh1, and sh2 CPSF3 knockdown cells. (F) Proliferation rates at days 0, 2, 4, and 6 of shNTC (blue), sh1 (orange), and sh2 (green) CPSF3 knockdown cells. (**) P < 0.01, (***) P < 0.001; two-way ANOVA with Dunnett's multiple comparisons test. (G) Mean tumor volume (mm3) of CPSF3 knockdown (orange) and control (blue) MiaPaCa2 tumors. (***) P < 0.001, two-way ANOVA.
