Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA

TABLE 3.

Sequences of protein and RNA constructs used in this study.

SPENRRM 1–4 MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRGGGGGPAYGPPPSLHAREGRYERRLDGSDSSSSSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREER
SPENRRM 2–4 FGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREER
SPENRRM 3–4 RTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREER
SPENRRM 2–4 mut3 FGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLAIGNLEKTTTYHDLRNIFQRFGEIVDIDIAKVNGVPQAAALQYCDIASVCKAIKKMDGEYLGNNRLKLGFGASMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREER
XISTA-full GGGCUGCGGAUACCUGGUUUUAUUAUUUUUUCUUUGCCCAACGGGGCCGUGGAUACCUGCCUUUUAAUUCUUUUUUAUUCGCCCAUCGGGGCCGCGGAUACCUGCUUUUUAUUUUUUUUUCCUUAGCCCAUCGGGGUAUCGGAUACCUGCUGAUUCCCUUCCCCUCUGAACCCCCAACACUCUGGCCCAUCGGGGUGACGGAUAUCUGCUUUUUAAAAAUUUUCUUUUUUUGGCCCAUCGGGGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUUUGAUUUUUUUUUUUCAUCGCCCAUCGGUGCU
XISTA1–4 GCUGCGGAUACCUGGUUUUAUUAUUUUUUCUUUGCCCAACGGGGCCGUGGAUACCUGCCUUUUAAUUCUUUUUUAUUCGCCCAUCGGGGCCGCGGAUACCUGCUUUUUAUUUUUUUUUCCUUAGCCCAUCGGGGUAUCGGAUACCUGCUGAUUCCCUUCCCCUCUGAACCCCCAACACUCUGGCCCAUCG
XISTA4–6 GCCGCGGAUACCUGCUUUUUAUUUUUUUUUCCUUAGCCCAUCGGGGUAUCGGAUACCUGCUGAUUCCCUUCCCCUCUGAACCCCCAACACUCUGGCCCAUCGGGGUGACGGAUAUCUGCUUUUUAAAAAUUUUCUUUUUUUGGCCCAUCGGGGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGG
XISTA5–8 GCCCAUCGGGGUGACGGAUAUCUGCUUUUUAAAAAUUUUCUUUUUUUGGCCCAUCGGGGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUU
XISTA6–8 GGCCCAUCGGGGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUU
XISTA7–8 GUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUU
XISTA6–9 GGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUUUGAUUUUUUUUUUUCAUCGCCCAUCGGUGCU
XISTA6–9+ GGCUUCGGAUACCUGCUUUUUUUUUUUUUAUUUUUCCUUGCCCAUCGGGGCCUCGGAUACCUGCUUUAAUUUUUGUUUUUCUGGCCCAUCGGGGCCGCGGAUACCUGCUUUGAUUUUUUUUUUUCAUCGCCCAUCGGUGCUUUUUAUGGAUGAAAAAAUGUUGGUUUUGUGGGUUGUUGCACUCUCUGGAAUAUCUACACUUUUUUUUGCUGCUGAUCAUUUGGUGG
SRA GUACCAGGUAUGUGAUGACAUCAGCCGACGCCUGGCACUGCUGCAGGAACAGUGGGCUGGAGGAAAGUUGUCAAUACCUGUAAAGAAGAGAAUGGCUCUACUGGUGCAAGAGCUUUCAAGCCACCGGUGGGACGCAGCAGAUGACAUCCACCGCUCCCUCAUGGUUGACCAUGUGACUGAGGUCAGUCAGUGGAUGGUAGGAGUUAAAAGAUUAAUUGCAGAAAAGAGGAGUCUGUUUUCAGAGGAGGCAGCCAAUGAAGAGAAAUCUGCAGCCACAGCUGAGAAGAACCAUACCAUACCAGGCUUCCAGCAGGCUUCAUAAUCCUCGGUUCCCCAGACUCACCGGACACCAUCUCCU
BARD1 GCGAGGAGCCUUUCAUCCGAAGGCGGGACGAUGCCGGAUAAUCGGCAGCCGAGGAACCGGCAGCCGAGGAUCCGCUCCGGGAACGAGCCUCGUUCCGCGCCCGCCAUGGAACCGGAUGGUCGCGGUGCCUGGGCCCACAGUCGCGCCGCGCUCGACCGCCUGGAGAAGCUGCUGCGCUGCUCGCGUUGUACUAACAUUCUGAGAGAGCCUGUGUGUUUAGGAGGAA
tRNACys GGGGGUAUAGCUCAGGGGUAGAGCAUUUGACUGCAAAUCAAGAGGUCCCUGAUUCAAAUCCAGGUGCCCCCUCCAAGCUUG

This Article

  1. Published in Advance December 26, 2023, doi: 10.1261/rna.079713.123 RNA 30: 240-255

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA