Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA

TABLE 2.

Kd of XIST constructs and control RNA binding to SPENRRM 1–4

RNA construct Kd (nM) Hill's coefficient
SRA 103 ± 16 2
XISTA-full 103 ± 31 1
XISTA1–4 71 ± 4 1
XISTA4-6 132 ± 10 2
XISTA5-8 127 ± 4 5
XISTA6-9 185 ± 9 2
XISTA6-9+ 48 ± 5 1
XISTA6-8 288 ± 39 1
XISTA7-8 248 ± 68 1
BARD1 (negative control) 223 ± 54 2
tRNACys (negative control) 245 ± 78 1

This Article

  1. Published in Advance December 26, 2023, doi: 10.1261/rna.079713.123 RNA 30: 240-255

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