Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA

TABLE 1.

Kd of XISTA-full, SRA, and BARD1 binding to SPEN protein constructs

Protein construct RNA Kd (nM) Hill's coefficient
SPENRRM 1–4 XISTA-full 100 ± 30 1
SPENRRM 2–4 XISTA-full 120 ± 30 1
SPENRRM 2–4 mut 3 XISTA-full 230 ± 40 1
SPENRRM 2–3 XISTA-full 1050 ± 110 2
SPENRRM 1–4 SRA 100 ± 20 2
SPENRRM 2–4 SRA 300 ± 30 2
SPENRRM 2–4 mut 3 SRA 950 ± 100 3
SPENRRM 2–3 SRA 5570 ± 700 1
SPENRRM 1–4 BARD1 210 ± 60 2
SPENRRM 2–4 BARD1 550 ± 30 10
SPENRRM 2–4 mut 3 BARD1 2300 ± 500 2
SPENRRM 2–3 BARD1 10,000 ± 0 2

This Article

  1. Published in Advance December 26, 2023, doi: 10.1261/rna.079713.123 RNA 30: 240-255

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