Dissection of protein and RNA regions required for SPEN binding to XIST A-repeat RNA

SPEN RRM 4 is required for binding to any of the RNA transcripts examined. (A) Diagram of SPEN_{RRM 2–4} and SPEN_{RRM 2–3} protein constructs. (B) Quantification of the bound fraction of SPEN_{RRM 2–4} and SPEN_{RRM 2–3} with XIST_A-full, SRA, or BARD1 RNA from EMSA experiments, n = 3. Data are the mean ± SEM. (C) Comparison of binding affinities for SPEN_{RRM 2–4} and SPEN_{RRM 2–3} for experimental and control RNA targets from replicate experiments, n = 3. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001. Data are the mean ± SEM.