Mutations in rpsU (bS21) increase the efficiency of prfB frameshifting in F. johnsoniae. (A) An example western blot, comparing the products generated from constructs FS-23 and C-23 in wild-type (WT) and mutant (ΔC, Y54A) strains of F. johnsoniae (as indicated). Annotations are as described in the legend to Figure 2. (B–E) Quantification of FE on mRNA from construct FS-M (B), FS-23 (C), FS-74 (D), FS-74-Wps (E), or FS-369-Wps (E), using method A (blue) and method B (green). Data represent the mean ± SEM. The number of biological replicates (n) is indicated below the bars, and the raw data are given in Supplemental Table S1. A two-tailed t-test was used to evaluate differences from WT. Uncorrected P-values: (*) P < 0.05; (**) P < 0.005; (***) P < 0.0005.
