Programmed prfB frameshifting is considerably less efficient in F. johnsoniae than in E. coli. (A) An example western blot, comparing products generated from constructs FS-23 and C-23 in F. johnsoniae (Fjo) and E. coli (Eco) cells, as indicated. Annotations are as described in the legend to Figure 2. (B,C) Quantification of FE on mRNA from constructs FS-M and FS-23 (as indicated), using method A (blue) and method B (green). Data represent the mean ± SEM. The number of biological replicates (n) is indicated below the bars, and the raw data are given in Supplemental Table S1. A two-tailed t-test was used to evaluate differences (Eco vs. Fjo). Uncorrected P-values: (*) P < 0.05; (**) P < 0.005; (***) P < 0.0005.
