FIGURE 7.
Impact of drugs affecting phosphorylation on the activity of SR proteins that repress CLK1 exon 4 inclusion. HCT116 cells transfected with plasmids allowing the expression of HA-SRSF10, HA-SRSF12, HA-SRSF3 or with an HA-EMPTY control plasmid were treated with DMSO, GPS167, TG003, and cantharidin. RNA was extracted after 48 h and analyzed by RT-PCR for CLK1 exon 4 inclusion. The ΔPSI values are plotted in the graph. Triplicates RT-PCR reactions fractionated on gels are shown in Supplemental Figure S6. (**) P < 0.01 and (***) P < 0.001, (n.s) not significant.
