Impact of gRNAs/dCas13Rx on CLK1 exon 4 splicing in HCT116 cells. (A) The positions on the CLK1 exon 4 alternative splicing unit of 25 nt-long gRNAs tested are indicated. The position of primers used for the RT-PCR is also shown. (B) Following transfection of the plasmids expressing the gRNA and dCas13Rx, RNA was extracted, and CLK1 exon 4 splicing was monitored by RT-PCR. (B) ΔPSI values that compare the impact of each guide relative to the gEMPTY plasmid are shown. Duplicates of the RT-PCR products fractionated on acrylamide gels are shown below the graph. The statistical significance of values was evaluated using multiple t-test analysis of technical triplicates. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001.
