Modulation of CLK1 exon 4 splicing by domains of SRSF3, TRA2β, and SRSF10. Plasmids allowing the expression of HA-tagged SRSF3 and TRA2β (A) or SRSF10 (B) proteins or portions thereof were transfected and tested for their impact on endogenous CLK1 exon 4 splicing. End point RT-PCR reactions were carried out to monitor CLK1 exon 4 inclusion. PSI values obtained for each sample were compared with the HA-EMPTY plasmid to obtain ΔPSI values, which are represented in the graph. Statistical significance was evaluated using multiple t-test analysis. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001; (ns) not significant. Gel duplicates for each sample displaying the RT-PCR reactions are shown in Supplemental Figure S1B.
