Effects of PQ on tRNA modification in wild-type cells. (A,B) HPLC chromatography of nucleosides from total tRNA isolated from wild-type (MJY1150) cells grown in the absence (control) or presence (0.25 or 2.5 mM) of PQ. The absorbance units (A.U.) were measured at 254 (A) or 240 nm (B). (C,D). Effects of PQ on the levels of indicated nucleosides/dinucleotide. The relative abundance was calculated by dividing the peak area of the relevant nucleoside/dinucleotide (at 254 nm) by the peak area for pseudouridine. The values represent the mean from three independent experiments. The standard deviation is indicated. Supplemental Table S2 includes values for other modified nucleosides. (*) P < 0.05, (**) P < 0.01, ns indicates not significant (P>0.05).
