Elevating microRNA levels by targeting biogenesis with steric-blocking antisense oligonucleotides

ASO-16 increases miR-1225 abundance in vivo. (A) Analysis of RNA isolated from kidneys of P21 mice treated with indicated ASO or vehicle control (C). miR-1225, miR-16 and sno65 were analyzed by radiolabeled stem–loop RT-PCR. Pkd1 and Gapdh were analyzed by radiolabeled RT-PCR. (–) refers to a control PCR reaction with no cDNA. Amplicons were separated by PAGE. (B) Quantitation of miR-1225 (left) normalized to miR-16 (top) or sno65 (bottom). (C) Quantitation of Pkd1 normalized to Gapdh. Error bars represent SEM, n = 3–7 mice. (*) P ≤ 0.05, one-way ANOVA with Dunnet's post hoc analysis samples compared to control.