Interactions between miR-1225 and PKD1 RNA expression. (A) miR-1225 and PKD1 are expressed in human tissues. Quantification of RT-PCR and stem–loop RT-PCR analysis of total human mRNA isolated from various tissues. The absolute amount of PKD1 mRNA and mature miR-1225 are calculated based on comparison to a standard curve generated by amplification of different concentrations of a synthetic sequence of known concentration and graphed as zmol/μg of total RNA. (B) The relative expression of miR-1225 to PKD1 is graphed as (zmol of miR-1225/μg of total RNA)/(zmol of PKD1/μg of total RNA). (C) Mouse miR-1225 and Pkd1 are dynamically expressed across the mouse life-span. Quantitation of radiolabeled stem–loop RT-PCR of mouse miR-1225 and RT-PCR of Pkd1 relative to sno65 and Gapdh, respectively (see Supplemental Fig. S1). Error bars represent standard error of the mean SEM. Postnatal day 2 (P2), n = 4; P23, n = 5; 7 months, n = 5; and 11–13 months, n = 6. Simple linear regression analysis indicates that the slopes are significantly different, P = 0.0002.
