Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-seq

TABLE 1.

The kobs values and amplitude parameters for MarathonRT NTA activity with dATP and dGTP in the standard RT buffer

Identity of NTA nucleotide No. of NTA nucleotide kobs (min−1) Amplitude
dATP 1 0.19 ± 0.02 96%
2 0.61 ± 0.18 96%
3 0.20 ± 0.03 86%
dGTP 1 0.035 ± 0.008 94%
2 0.040 ± 0.015 80%
  • The parameters were calculated from the average values of three independent experiments along with standard errors.

This Article

  1. RNA 30: 1495-1512