Sequencing read length and isoform detection comparison of uMRT and Maxima on poly(A) selected RNAs extracted from Huh7.5 cell line using ONT long-read sequencing. (A) Boxplots of sequencing read length in bp across replicate 1 and replicate 2 of uMRT and Maxima, respectively. Statistical significance was assessed using a two-sided Wilcoxon rank-sum test. Center line indicates the median, box limits indicate the upper and lower quantiles, and whiskers indicate the 1.5× interquartile range. (B) Bar charts depicting the mean number of isoforms detected (CPM > 0) between both replicates of uMRT and Maxima, respectively, with reads downsampled to 1.2 million for every individual sample to account for sequencing depth.
