Comparison of gene detection by uMRT and SSII on universal human reference total RNA using Illumina short-read sequencing. (A) Pie charts depicting the proportional distribution of mapped sequencing reads across genomic features captured by uMRT (left) and SSII (right), respectively. Read assignment and mapping were both performed using the human reference GRCh38 (RefSeq GRCh38.p14, GCF_000001405.40, primary assembly) with STAR (v2.7.11b) and RSeQC (v5.0.1). Pie slices are colored according to genomic features. (B) Bar charts illustrating the mean number of genes detected (TPM > 0) between both replicates of uMRT and SSII, respectively, with reads downsampled to 20 million for every individual sample to account for sequencing depth. Colors indicate the gene biotype of the detected genes depicting the distribution across all detected biotypes (left) and zoomed in on non-protein-coding genes (right).
