Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-seq

Examining the performance of ERCC RNA-seq library prepared using the template-switching reaction of uMRT. (A) Linear regression to examine the correlation between ERCC RNA transcript concentration (x-axis) used in library preparation and the normalized read count (TPM) (y-axis) calculated from the sequencing reads. The TPM values and ERCC transcript concentrations were log2 transformed. (B) Examining the reproducibility of RNA-seq experiment by comparing the normalized ERCC read count (log2 transformed TPM) of two repeated experiments.