Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-seq

Evaluating the impact of RNA template 5′-nucleobase identity and 5′-modification on MarathonRT template switching efficiency. (A) Outline of the experimental design to analyze the impact of RNA template 5′-nucleobase identity and 5′-modifications on template-switching efficiency. The RNA template 5′-nucleobase identity and 5′-modifications are described in the legend of the figure. (B) Quantification of the template-switching efficiency using the RNA templates with different 5′-nucleobase identity and 5′-modifications. Each reaction was repeated three times with mean and standard deviation shown in the figures.