BC120 overexpression negatively regulates the translation of a GFP reporter. (A) MCF-7 cells were transfected with a GFP reporter plasmid that contained an expression cassette for the various indicated RNAs. Twenty-four hours posttransfection, cells were harvested, and total cell extracts were collected and normalized for total protein content. GFP was quantified by fluorescence measurements of 20 µL cell lysate in a 96-well plate. Data represent the mean of 12 replicates +/− standard error. Statistical significance was assessed by performing a one-way ANOVA followed by Dunnett's multiple comparison test (GraphPad Prism software). (B) Total RNA extracted from a set of samples used in (A) was separated by denaturing TBE gel electrophoresis, and northern blot was performed with a probe targeting BC200 nt 33–52.
