Knockdown of BC120 and BC200 with Alu domain targeting LNA GapmeRs. (A) MCF-7 cells were transfected with the indicated LNA GapmeRs. After 48 h, total RNA was extracted for northern blot with a probe targeting nt 33–52. (B) Twenty-five nanograms of RNA from the samples in (A) was used as a template for RT-qPCR with primer/probe sets to quantify expression of BC120, BC200, and 7SL. Data are relative to the untreated cells and represent the mean of three replicates +/− SD. (C) MCF-7 cells were transfected as in (A) and counted at the indicated time points. Data represent the calculated total number of cells in each well and is the mean of four wells per condition +/− SD. (D) As in (A), to the same samples a cell impermeable viability dye (DAPI) was added to each sample, and the proportion of DAPI positive cells was measured at each time point for each condition. Data represent the mean of four wells per condition +/− SD.
