FIGURE 6.
BC200 expression is reduced at high cell density. (A) MCF-7 cells were plated at the indicated cell densities and collected at the indicated times before total RNA extraction. Five micrograms of total RNA was separated by denaturing TBE-urea PAGE, and northern blot performed with a probe targeting BC200 nt 33–52. Blot is representative of three replicate samples. (B) Band intensities in (A) were quantified by densitometry and plotted relative to the 24 h sample. Data represent the mean of three replicates +/− SD.
