The BC200 truncation is expressed in a broad spectrum of immortalized cell lines and normal human tissue. (A) Indicated cell lines were transfected with a plasmid containing a BC200 expression cassette. After 48 h, 5 µg of RNA from the indicated cells was used for northern blot with a probe targeting BC200 nt 63–84. (B) A series of BC200 targeting LNA oligonucleotide probes were assessed for specificity using MCF-7 cells that were untransfected (UT), transfected with a BC200 expression plasmid (BC200) or empty vector (EV). Targeted region of BC200 is indicated below each blot. (C) Endogenous BC200 was assessed by northern blot using 5 µg total RNA collected 48 h after plating. (D) Two micrograms of total RNA from the indicated normal human tissues was northern blotted with a probe targeting BC200 nt 33–52. In the case of Brain, 500 ng total RNA was loaded to balance the signal intensity.
