Analysis of BC120 expression in tubo-ovarian HGSC. (A) Twenty-five nanograms of RNA from the indicated samples was used as a template for RT-qPCR using a full-length Taqman primer set for BC200 and the optimized qPCR assay for BC120. Data represent the mean of three replicates for BC200 and four replicates for BC120 +/− SD. EOC ### samples are ovarian cancer samples derived from the ascites fluid of individual patients. PEO1, PEO4, and PEO6 are HGSC cell lines derived from a single patient. FT194 and FT246 are immortalized fallopian tube secretory epithelial cells, and CAOV3 is an HGSC cell line. (B) Northern blot of samples used in (A) for which sufficient RNA quantity was available using a probe complementary to nt 33–52 of the BC200 RNA. In the left panel, 2 µg of RNA was loaded for each sample, and in the right panel, 750 ng of RNA was loaded for each sample due to sample quantity limitations.
