An ∼119 nt RNA is coexpressed with BC200. (A, left panel) T-47D cells were transfected with a plasmid containing a BC200 expression cassette or empty vector (Ctrl). RNA was extracted at the indicated time points. Northern blot was performed with 5 µg of total RNA with a probe complementary to BC200 nt 63–84. (Right panel) T-47D cells were transfected with plasmids containing expression cassettes for BC200 and the indicated truncations. All truncations begin with the first nucleotide of BC200 and are terminated by the insertion of four consecutive thymidines in the coding sequence after the indicated base. In total, 10 fmoles of in vitro transcribed BC119 serves as a size marker. Northern blot was performed with a probe complementary to BC200 nt 63–84. (B) Proposed secondary structure of the BC200 RNA.
