Abolished frameshifting for predicted structure-stabilizing SARS-CoV-2 mutants: implications to alternative conformations and their statistical structural analyses

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FIGURE 4.
FIGURE 4.

Conformational flexibility and frameshifting efficiency of FSE in larger constructs. Arc plot and radial layout computed by DMS-MaP and SHAPEknots for (A) 156 nt construct in 3_5, 2_1, and 3_5* topology and for (B) 222 nt construct in 2_1 and 3_5* topology. Slippery site, Stem I, Stem II, and Stem III are annotated in the arc plot and radial layout. (D) Frameshifting efficiency of 156 nt and 222 nt construct stabilizing mutants highlighting substantial increase in frameshifting efficiency when compared to WT 87 nt FSE construct. Frameshifting efficiency was calculated using dual-luciferase assays.

This Article

  1. Published in Advance July 31, 2024, doi: 10.1261/rna.080035.124 RNA 30: 1437-1450

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