DMS-MaP and frameshifting efficiency of FSE conformation stabilizing mutants. Arc plot and radial layout computed by DMS-MaP and SHAPEknots for (A) FSE-3_6 mutants stabilizing 3_6 H-type pseudoknots, (B) FSE-3_5 mutants stabilizing 3_5 three-way junctions, and (C) FSE-3_3 mutants stabilizing 3_3 H-type pseudoknots. Stem I, Stem II, and Stem III are annotated in the arc plot and radial layout. Arrows in arc plot and asterisks in radial layout represent point mutations. (D) Frameshifting efficiency of 3_6, 3_5, and 3_3 stabilizing mutants highlighting substantial decrease in frameshifting efficiency when compared to WT 87 nt FSE construct. Frameshifting efficiency was calculated using dual-luciferase assays.
