Abolished frameshifting for predicted structure-stabilizing SARS-CoV-2 mutants: implications to alternative conformations and their statistical structural analyses

DMS-MaP for crystal structure constructs of SARS-CoV-2 FSE. (A) 3D crystal structure (PDB ID 7LYJ) of 66 nt FSE construct in 3_6 H-type pseudoknot conformation. (B) Arc plot and radial layout of 7LYJ computed through DMS-MaP and SHAPEknots in 3_6 topology. (C) 3D crystal structure (PDB ID 7MLX) of 65 nt FSE construct in 3_6 H-type pseudoknot conformation. (D) Arc plot and radial layout of 7MLX computed through DMS-MaP and SHAPEknots in 3_6 topology. A minor 3_1 conformation for 7MLX is also present. Stem I, Stem II, and Stem III are annotated in the arc plot and radial layout.