TABLE 2.
Single turnover multiple nucleotide incorporation rates (kobs) for Thg1/TLP activity
| Enzyme | 8 bpa (min−1) | 7 bpa (min−1) | 5′-truncated tRNA (min−1) | Full-length tRNAHisA73 (min−1) |
|---|---|---|---|---|
| ScThg1 | ND | ND | ND | 3.6 ± 0.3 |
| AcaTLP2 | 0.63 ± 0.06 | 0.45 ± 0.02 | 4.9 ± 2.2 | NT |
| DdiTLP4 | 1.9 ± 0.1 | 3.5 ± 0.1 | 0.71 ± 0.05 | NT |
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(ND) No activity detected for this substrate; (NT) not tested.
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a Average percent product conversion at each time point was calculated from three independent experiments, plotted vs. time and fit to a single exponential rate equation to calculate kobs using Equation 1.
