TABLE 1.
KD values for Thg1/TLP binding to RNA duplexes measured by filter-binding assay
| RNA | AcaTLP2a (µM) | DdiTLP4a (µM) | MsTLPa (µM) | BtTLPa (µM) | ScThg1b (µM) |
|---|---|---|---|---|---|
| 19 bp | 0.17 ± 0.02 | 1.0 ± 0.3 | 8.0 ± 2.4 | 6.8 ± 1.5 | 0.065 ± 0.008 |
| 13 bp | 0.18 ± 0.04 | 0.2 ± 0.1 | 3.9 ± 0.8 | 12.1 ± 4.1 | 0.042 ± 0.002 |
| 10 bp | 0.21 ± 0.05 | 0.6 ± 0.2 | 2.1 ± 0.3 | 13.5 ± 2.2 | 0.061 ± 0.008 |
| 8 bp | 0.19 ± 0.03 | 0.2 ± 0.1 | 3.5 ± 0.6 | 9.7 ± 1.2 | 0.075 ± 0.008 |
| 7 bp | 0.16 ± 0.03 | 0.7 ± 0.1 | 3.7 ± 0.8 | 11.5 ± 4.3 | 0.054 ± 0.010 |
| Mx-tRNAHis | 5.88 ± 3.12 | >10c | 1.7 ± 0.2 | >10c | 2.6 ± 0.5 |
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aAverage fraction bound at each enzyme concentration was calculated from three independent experiments, plotted vs. enzyme concentration to calculate KD using Equation 2.
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bKD values for ScThg1 were calculated using only two independent experiments.
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cA lower limit to KD was estimated for DdiTLP4 and BtTLP based on the highest enzyme concentration used in the assays.
