TABLE 2.
Binding kinetic parameters of the interaction of IFIT1, IFIT1/IFIT3, or IFIT1/IFIT2/IFIT3 with differently capped mRNAs
| Ligand-nt16RNA | Protein | KD (nM) | Ka (103·M−1·sec−1) | kd (10−3·sec−1) |
|---|---|---|---|---|
 |
IFIT1 | 3.54 | 65 ± 0.67 | 0.21 ± 0.01 |
| IFIT1/IFIT3 | 0.55 | 149 ± 1.7 | 0.081 ± 0.01 | |
| IFIT1/IFIT2/IFIT3 | n.d. | n.d. | n.d. | |
 |
IFIT1 | 23.3 | 147 ± 1.44 | 3.43 ± 0.03 |
| IFIT1/IFIT3 | 2.06 | 202 ± 2.4 | 0.417 ± 0.01 | |
| IFIT1/IFIT2/IFIT3 | n.d. | n.d. | n.d. | |
 |
IFIT1 | 114.4 | 68.8 ± 4.46 | 7.9 ± 0.16 |
| IFIT1/IFIT3 | 34.04 | 109 ± 2.05 | 3.4 ± 0.06 | |
| IFIT1/IFIT2/IFIT3 | 17.5 | 96.2 ± 1.84 | 1.56 ± 0.05 | |
| m7 GpppAG_m6A m7 GpppAG_Gm6AGm6ACCGGCCTCGm6A |
IFIT1 | 1.49 | 102 ± 1.29 | 0.15 ± 0.01 |
| IFIT1/IFIT3 | 0.43 | 105 ± 1.57 | 0.047 ± 0.02 | |
| IFIT1/IFIT2/IFIT3 | n.d. | n.d. | n.d. |
-
Displayed KD, kd, and ka values represent the average of three replicate experiments. (n.d.) Not determined.
